(Born October 8, 1515- Died March 7, 1578)
Daughter of Archibald Douglas, 6th Earl of Angus and Margaret Tudor
Married to Matthew Stewart, 4th Earl of Lennox
Mother of Henry Stuart, Lord Darnley and Charles Stuart, 1st Earl of Lennox

Margaret Douglas was the daughter of the dowager Queen of Scotland Margaret Tudor. She incurred her uncle Henry VIII’s wrath twice; the first time was for her unauthorised engagement to Lord Thomas Howard and the second was in 1540 for an affair with Thomas Howard’s nephew Sir Charles Howard, the brother of Henry’s wife Katherine Howard. Her son Henry Stuart, Lord Darnley, was married to Mary Queen of Scots and was the father of James VI of Scotland (also known as James I of England).

Margaret Douglas was born on October 8, 1515 at Harbottle Castle in Northumberland. Her mother was Margaret Tudor, the Dowager Queen of Scotland and the sister of Henry VIII, and her father was her mother’s second husband Archibald Douglas, 6th Earl of Angus. Margaret Tudor had recently been forced to hand over the Scottish Regency to the Duke of Albany, who had arrived from France, and she was forced to flee to England. Margaret Tudor arrived in London with baby Margaret on May 3, 1516, while her husband was dealing with issues in Scotland. When Albany returned to France on June 6 , 1517, the Queen Dowager was permitted to return and was given limited access to see her son, James V, at Edinburgh Castle. During this time, she had a falling out with her husband and Angus took custody of Margaret Douglas. When Margaret was not living with her father, she stayed with her godfather Cardinal Wolsey.

When Wolsey died in 1530, Lady Margaret was invited to the royal Palace of Beaulieu, where she resided in the household of Princess Mary. Because of her nearness to the English crown, Lady Margaret Douglas was brought up chiefly at the English court in close association with Mary, her first cousin, the future Queen Mary I, who remained her lifelong friend. Margaret would later become first lady-in-waiting to Anne Boleyn and Lady-of-Honour to Princess Elizabeth. Yet, when Margaret became secretly betrothed to Sir Thomas Howard, Anne Boleyn’s uncle and Norfolk’s youngest brother, Henry VIII, in July 1536, placed them both in the Tower. Margaret did fall ill while in the Tower. Margaret was released on October 29, 1537, but Sir Thomas died in the Tower on October 31, 1537.

In 1539, Margaret was part of the group of people who was supposed to meet Anne of Cleves at Greenwich Palace and join her household, but Henry changed his mind and met Anne of Cleves at Rochester instead. In 1540, Margaret was again in disgrace with the King when she had an affair with Lord Thomas Howard’s half-nephew Sir Charles Howard. He was the son of Thomas’ elder half-brother Lord Edmund Howard, and a brother of Henry VIII’s fifth wife, Katherine Howard. Her mother, Margaret Tudor, died at Methven Castle on October 18, 1541 from palsy. Margaret would be one of the few witnesses to King Henry VIII’s last marriage to Katherine Parr, in 1543; Margaret was a close friend to Katherine Parr and would become one of her chief ladies.

In 1544, Lady Margaret married a Scottish exile named Matthew Stewart, 4th Earl of Lennox, who would later became regent of Scotland. Their children were Henry Stuart, Lord Darnley and Charles Stuart. When Mary I became queen in 1553, Margaret returned to court and was given rooms in Westminster Palace. Margaret would be one of the chief mourners at Mary’s funeral in 1558 and when Elizabeth I became queen, Margaret moved to Yorkshire, where her home at Temple Newsam became a center for Roman Catholic intrigue.

Margaret succeeded in marrying her elder son, Lord Darnley, to his first cousin, Mary, Queen of Scots, thus uniting their claims to the English throne. Queen Elizabeth I disapproved of this marriage and had Margaret sent to the Tower of London in 1566. After the murder of Margaret’s son Darnley in 1567, Margaret was released from prison and she was the first to denounce her daughter-in-law, but was eventually later reconciled with her. Her husband assumed the government of Scotland as regent, but was assassinated in 1571. Margaret would never marry again.

In 1574, she again aroused Queen Elizabeth’s anger by marrying her younger son Charles to Elizabeth Cavendish, the daughter of Sir William Cavendish and Bess of Hardwick and the stepdaughter of the Earl of Shrewsbury. She was again sent to the Tower, unlike the Countess of Shrewsbury, but was pardoned after her son Charles’ death in 1576. Margaret would take care of Charles’ daughter Arbella Stuart until her own death on March 7, 1578.

Sources:
https://en.wikipedia.org/wiki/Margaret_Douglas
http://www.maryqueenofscots.net/people/lady-margaret-douglas-countess-lennox/
https://www.britannica.com/biography/Margaret-Douglas-Countess-of-Lennox